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ARMS-PCR design
HRM Assay Design (dbSNP)
HRM Assay Design
HRM Assay Design(Indel)
High Resolution Melt (HRM) – Oligo Design Tool
Upstream Sequence (5'>3') - Max:100 bases
Allele 1
A
T
G
C
Allele 2
<
C
G
T
A
Downstream Sequence (5'>3') - Max:100 bases
[Mg
2+
]
mM
GC%:
CLEAR
Tm(
0
C):
